The lipidomic research is currently devoting considerable effort to the harmonization that should enable the generation of comparable and accurate quantitative lipidomic data across different laboratories and regardless of the mass spectrometry-based platform used. In the present study, we systematically investigate the effects of the experimental setup on quantitative lipidomics data obtained by two lipid class separation approaches, hydrophilic interaction liquid chromatography (HILIC) and ultrahigh-performance supercritical fluid chromatography (UHPSFC), coupled to two different quadrupole - time of flight (QTOF) mass spectrometers from the same vendor. This approach is applied for measurements of 268 human plasma samples of healthy volunteers and renal cell carcinoma patients resulting in four data sets. We investigate and visualize differences among these data sets by multivariate data analysis methods, such as principal component analysis (PCA), orthogonal partial least square discriminant analysis (OPLS-DA), box plots, and logarithmic correlations of molar concentrations of individual lipid species. The results indicate that even measurements in the same laboratory for the same samples using different analytical platforms may yield slight variations in the molar concentrations determined. The normalization to a reference sample with defined lipid concentrations can further diminish these small differences, resulting in highly homogenous molar concentrations of individual lipid species. This strategy indicates a potential approach towards the reporting of comparable quantitative results independent from the quantitative approach and mass spectrometer used, which is important for a wider acceptance of lipidomics data in various biomarker inter-laboratory studies and ring trials.Antibiotics have made great contributions to the improvement of human health and life quality. However, the current abuse of antibiotics not only has a serious impact on the environment, but also endangers people's health. For this reason, the simultaneous identification and accurate determination of as many antibiotics in the environment, food and organisms as possible is critical. Herein, a ratiometric fluorescent sensor array based on Eu3+ and Tb3+ co-doped metal-organic frameworks (MOFs) was fabricated. https://www.selleckchem.com/products/stat3-in-1.html Benefiting from the sensitization of the organic ligands to Eu3+ and Tb3+, the reaction of MOFs with various antibiotics resulted in different responses to the ratio of fluorescent intensity at 545 nm and 616 nm (F545/F616). After these responses were differentiated by principal component analysis (PCA), totally eight kinds of 25 antibiotics were well distinguished with the existence of interfering substances. The proposed sensor array exhibited high accuracy (98%) for the identification of 48 unknown samples in water and outstanding quantitative ability for the mixture of antibiotics. Finally, the practicability of the sensor array for the analysis of real samples was proved. In this strategy, we have not only provided an efficient way for the comprehensive identification and determination of antibiotics, but also promised new opportunities for the development of ratiometric signal based sensor array.Fluorescent probes containing 1,8-naphthalimide dyes have been used to detect biomolecules in the environmental and biological fields. However, most of the probes only exhibit single fluorescent output to one analyte, making them insufficient for detection of more analytes. Herein, we developed a novel 1,8-naphthalimide-based lysosome-targeting dual-analyte sensitive fluorescent probe (DPPP) for the detection of pH and palladium (Pd0) using two different emissive channels. The probe showed high selectivity, large Stokes shifts (Δλ ? 100 nm) and enhanced response to pH, with blue emission at 485 nm via a morpholine group, and responsive to Pd0 concentration, with yellow emission at 545 nm via an allylcarbamate group. The effect of DPPP was successfully observed for sensitive visualizing pH and Pd0 concentration in the lysosome of HeLa cells and zebrafish using fluorescence microscopy. This work provides guidance for the design of dual-analyte fluorescent probes.Inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) was investigated for possible use in food fraud studies through the measurement of strontium and sulfur isotope ratios. Oxygen mass shift mode was applied to shift 87Sr/86Sr and 34S/32S isotope ratios to their respective oxides, 87Sr16O+/86Sr16O+ and 34S16O+/32S16O+, effecting a gas-phase chemical separation of the elements from Rb and Kr (for Sr) and molecular N and O species, along with P- and S-hydrides (for S). A total least squares regression approach was employed to generate the isotope ratio data from time-resolved analyses, and method uncertainties and accuracies were determined. The utility of the approach was shown by using the Sr and S isotope ratios together to differentiate between NIST RM 8256 Wild-Caught Coho Salmon and NIST RM 8257 Aquacultured Coho Salmon. These materials are currently under development at NIST as certified food fraud standards and method evaluation materials for comprehensive chemical analysis.A microfluidic colorimetric detection (MCD) platform consisting of a sliding hybrid PMMA/paper microchip and a smart analysis system is proposed for the convenient, low-cost and rapid analysis of human urine and whole blood samples. The sliding PMMA/paper microchip comprises a PMMA microfluidic chip for sample injection and transportation, a paper strip for sample filtration (urine) or separation (blood), and a sealed paper-chip detection zone for sample reaction and detection. In the proposed device, the paper-chip is coated with bicinchoninic acid (BCA) and biuret reagent and is then assembled into the PMMA microchip and packaged in aluminum housing. In the detection process, the PMMA/paper microchip is slid partially out of the housing, and 2 μL of sample (urine or whole blood) is dripped onto the sample injection zone. The chip is then slid back into the housing and the sample is filtered/separated by the paper strip and transferred under the effects of capillary action to the sealed paper-chip detection zone.